#  Colony PCR 

 



###  Preparation 

 Streak out yeast and grow until small colonies form.

###  Procedure 

 Place 5 ul of water into a PCR tube.

 Stick a pipette tip into the colony to pick up a small number of cells. Smash the pipette tip into the cells.

 Freeze the PCR tube for 10-15 min.

 Microwave the PCR tube for 1 min on high power.

 Freeze the PCR tube for 10-15 min.

 Prepare 45 ul of the PCR mix.

 Add the PCR mix to the tube, mix, spin down, and PCR as normal.