@article {1472710,
	title = {Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosis},
	journal = {PLOS Biology},
	volume = {18},
	number = {3},
	year = {2020},
	abstract = {Different members of the same protein family often perform distinct cellular functions. How much are these differing functions due to changes in the biochemical activity of a protein itself versus changes in other proteins? We asked how the budding yeast, Saccharomyces cerevisiae, evolves when forced to use the meiosis-specific kleisin, Rec8, instead of the mitotic kleisin, Scc1, during the mitotic cell cycle. This perturbation impairs sister chromosome linkage and reduces reproductive fitness by 45\%. We evolved 15 populations for 1750 generations, substantially increasing their fitness, and analyzed their genotypes and phenotypes. We found no mutations in Rec8, but many populations had mutations in the transcriptional mediator complex, cohesin-related genes, and cell cycle regulators that induce S phase. These mutations improve sister chromosome cohesion and slow genome replication in Rec8-expressing cells. We conclude that changes in known and novel partners allow proteins to improve their ability to perform new functions.},
	url = {https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000635},
	author = {Yu-Ying Phoebe Hsieh and Makrantoni, Vasso and Daniel Robertson and Adele L Marston and Murray, Andrew W}
}
