The role of cyclin synthesis and degradation in the control of maturation promoting factor activity

A W Murray; M J Solomon; M W Kirschner

doi:10.1038/339280a0

The role of cyclin synthesis and degradation in the control of maturation promoting factor activity

Nature. 1989 May 25;339(6222):280-6. doi: 10.1038/339280a0.

Authors

A W Murray¹, M J Solomon, M W Kirschner

Affiliation

¹ Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143-0448.

PMID: 2566918
DOI: 10.1038/339280a0

Abstract

We show that cyclin plays a pivotal role in the control of mitosis. A proteolysis-resistant mutant of cyclin prevents the inactivation of maturation promoting factor and the exit from mitosis both in vivo and in vitro. We have used a fractionated extract to study the activation of MPF by added cyclin protein.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Cell Cycle*
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / physiology
Female
Growth Substances / metabolism*
Kinetics
Maturation-Promoting Factor
Meiosis
Mitosis
Nuclear Proteins / biosynthesis
Nuclear Proteins / metabolism*
Ovum / cytology
Proliferating Cell Nuclear Antigen
Xenopus

Substances

Growth Substances
Nuclear Proteins
Proliferating Cell Nuclear Antigen
Maturation-Promoting Factor