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Genetic drift and selection in many-allele range expansions

Journal Article

We experimentally and numerically investigate the evolutionary dynamics of four competing strains of E. coli with differing expansion velocities in radially expanding colonies. We compare experimental measurements of the average fraction, correlation...

The cell cycle: an introduction

Book

In the last decade there has been a revolution in our comprehension of how cells grow and divide. Results from experiments on yeast, embryos, and cultured mammalian cells have unified seemingly disparate viewpoints into a single set of principles for...

Studies on intercellular LETS glycoprotein matrices

Journal Article

Intercellular matrices secreted by chick embryo fibroblasts in culture were studied by scanning electron microscopy. Cell-cell contact is a prerequisite for the expression of such matrices. The smallest fiber detected by transmission electron microscopy...

Construction of artificial chromosomes in yeast

Journal Article

Fifty-five-kilobase long artificial chromosomes containing cloned genes, replicators, centromeres and telomeres have been constructed in yeast. These molecules have many of the properties of natural yeast chromosomes. Centromere function is impaired on...

Pedigree analysis of plasmid segregation in yeast

Journal Article

We have used pedigree analysis to investigate the mitotic segregation of circular and linear DNA plasmids in Saccharomyces cerevisae. Circular ARS plasmids, which bear putative chromosomal replication origins, have a high segregation frequency and a...

Cloning, sequencing, and functional analysis of oriL, a herpes simplex virus type 1 origin of DNA synthesis

Journal Article

The herpes simplex virus type 1 genome (160 kilobases) contains three origins of DNA synthesis: two copies of oriS located within the repeated sequences flanking the short unique arm (US), and one copy of oriL located within the long unique arm (UL)...

Chromosome length controls mitotic chromosome segregation in yeast

Journal Article

We have examined the effect of physical length on the mitotic segregation of artificial chromosomes and fragments of natural yeast chromosomes. Increasing the length of artificial chromosomes decreases the rate at which they are lost during mitosis. We...

Construction and behavior of circularly permuted and telocentric chromosomes in Saccharomyces cerevisiae

Journal Article

We developed techniques that allow us to construct novel variants of Saccharomyces cerevisiae chromosomes. These modified chromosomes have precisely determined structures. A metacentric derivative of chromosome III which lacks the telomere-associated X...

An alternative pathway for meiotic chromosome segregation in yeast

Journal Article

In meiosis I of most organisms, homologous chromosomes pair, recombine, and then segregate to opposite poles of the cell. Crossing-over is normally necessary to ensure the proper segregation of the homologs. Recently developed techniques have made it...

Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae

Journal Article

We have examined the replication and segregation of the Saccharomyces cerevisiae 2 microns circle. The amplification of the plasmid at low copy numbers requires site-specific recombination between the 2 microns inverted repeat sequences catalyzed by the...

Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae

Journal Article

We have investigated two reactions that occur on telomeric sequences introduced into Saccharomyces cerevisiae cells by transformation. The elongation reaction added repeats of the yeast telomeric sequence C1-3A to telomeric sequences at the end of linear...

Cyclin synthesis drives the early embryonic cell cycle

Journal Article

We have produced extracts of frog eggs that can perform multiple cell cycles in vitro. Destruction of the endogenous messenger RNA arrests the extracts in interphase. The addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles. The...