Publications

The CCT chaperonin uses a specific ring topology of eight related subunits to instruct tubulin folding by site-specific interactions. Subunits comprising the negatively-charged hemisphere (Cct2/4/5/7) have the potential to self-assemble but how the...

Fast glycolytic growth in the budding yeast, Saccharomyces cerevisiae, produces two epigenetic states: arrestors, which primarily ferment, and recoverers, which respire. Positive feedback in mitochondrial translation produces the two states: mitochondrial...

In meiosis, one round of DNA replication followed by two rounds of chromosome segregation halves the ploidy of the original cell. Accurate chromosome segregation in meiosis I depends on recombination between homologous chromosomes. Sister centromeres...

Eukaryotic translation elongation factor 1A (eEF1A) is a highly abundant, multi-domain GTPase. Post-translational steps essential for eEF1A biogenesis are carried out by bespoke chaperones but co-translational mechanisms tailored to eEF1A folding remain...

Evidence suggests that increases in ploidy have occurred frequently in the evolutionary history of organisms, and can serve adaptive functions to specialized, somatic cells in multicellular organisms (Edgar & Orr-Weaver, 2001; Orr-Weaver, 2015; Van De...

In meiosis, one round of DNA replication followed by two rounds of chromosome segregation halves the ploidy of the original cell. Accurate chromosome segregation in meiosis I depends on recombination between homologous chromosomes. Sister centromeres...

 

Cell division without cell separation produces multicellular clusters in budding yeast.

Two fundamental characteristics of these clusters are their size (the number of cells

per cluster) and cellular composition: the fractions of cells with different...

The mating of fungi depends on pheromones that mediate communication between two mating types. Most species use short peptides as pheromones, which are either unmodified (e.g., a-factor in Saccharomyces cerevisiae) or C-terminally farnesylated (e.g., a-f...

The conserved regulon of Heat Shock Factor 1 in budding yeast contains chaperones

for general protein folding as well as Zinc Finger Protein Zpr1, whose essential role in

archaea and eukaryotes remains unknown. Here, we show that Zpr1 depletion causes

ac...

The evolution of complex multicellularity opened paths to increased morphological diversity and organizational novelty. This transition involved three processes: cells remained attached to one another to form groups, cells within these groups...

Comparisons of genomes of different species are used to identify lineage-specific genes, those genes that appear unique to one species or clade. Lineage-specific genes are often thought to represent genetic novelty that underlies unique adaptations...

In haploid budding yeast, evolutionary adaptation to constitutive DNA replication stress alters three genome maintenance modules: DNA replication, the DNA damage checkpoint, and sister chromatid cohesion. We asked how these trajectories depend on genomic...